5. The trocar-cannula, with the push rod inside, is placed 4-7 inches (10-15cm) behind the end of the xiphoid (breastbone), and 2-3 inches (5-7cm) to the right of the midline.

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Perforate the abdominal wall and the abomasum with a swift downward push of the trocar.

BE CAREFUL TO AVOID THE LARGE SUBCUTANEOUS ABDOMINAL VEINS IN THIS AREA!

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(Pre-marking with a colored marking stick helps to locate the veins. However, should a vein be accidentally perforated, a simple interrupted suture can be placed at the site to minimize any bleeding.) The handle is removed from the cannula, and the push rod is run through the center of it to remove any skin plug, which often is present.

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6. The distinct odor of abomasal gas may be identified at this time, and if desired, a small amount of fluid can be extracted via small diameter plastic tubing and checked for low pH (2-4).

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